BPI-China #

Detection of mutiple miRNAs based on isothermal amplification on colloidal gold and CRISPR technology #

Myocardial infarction is a common disease worldwide. There are approximately 330 million current heart attack victims in China. miRNA was considered as one of the most potential new biomarkers of myocardial infarction.Therefore, the development of miRNA detection methods is of great significance. The design idea of this project was to modify the hairpin structure on the surface of colloidal gold particles, and then the target miRNA could open the hairpin structure to initiate isothermal amplification reaction. By constructing Cas12a and crRNA vectors, the reverse cleavage function of CRISPR technology was used to release fluorescent signals and complete miRNA detection. This project would establish a fast and convenient miRNA detection method based on CRISPR, helping people to conveniently and efficiently detect miRNAs and contribute to myocardial infarction prevention.

心筋梗塞は世界中で共通の病気です。中国には現在約3億3000万人の心臓発作の被害者がいます。miRNAは心筋梗塞の最も有望な新たなバイオマーカーの1つと考えられています。従って、miRNA検出方法の開発は大きな意義があります。このプロジェクトの設計思想は、コロイド金粒子の表面のヘアピン構造を修正し、その後ターゲットとなるmiRNAがヘアピン構造を開いて等温増幅反応を開始するというものでした。Cas12aとcrRNAベクターを構築することで、CRISPR技術の逆切断機能を用いて蛍光信号を放出し、miRNA検出を完了する。このプロジェクトは、CRISPRに基づく迅速で便利なmiRNA検出方法を確立し、人々が便利かつ効率よくmiRNAを検出し、心筋梗塞の予防に貢献することになるでしょう。

reference:

• iGEM BPI-China Team Information
• iGEM BPI-China Wiki