October 21, 2023
Ohio-State-FABE # DiGI-T3 - Digitally Optimized Gastrointestinal Therapeutics for Type 3 Secretion # Many diseases result from dysfunctional proteins, but therapeutics directly targeting them are limited. Current treatments, mainly small molecules, cause side effects. Ohio State’s iGEM team is exploring a solution using Type III Secretion Systems (T3SS) for continuous therapeutic protein delivery. Although native to some infectious bacteria, T3SS can be modified for safe use in Escherichia coli to deliver therapeutic proteins, particularly targeting gastrointestinal diseases.
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October 21, 2023
Paris-Bettencourt # Lubritect # Sexually transmitted infections (STIs)is a serious public health issue worldwide: an alarming incidence (1M new infections/day), prevalence (80% of sexually active individuals acquire human HPV by 45) and disease burden (82,000 deaths/year from hepatitis B).
性感染症（STIs）は、世界的に深刻な公衆衛生の課題です：驚異的な発生率（1日に新たに100万人が感染）、有病率（性的に活動的な個人の80%が45歳までにヒトパピローマウイルスを取得）および疾病負荷（肝炎Bによる年間82,000人の死亡）。
reference:
iGEM Paris-Bettencourt Team Information iGEM Paris-Bettencourt Wiki
October 21, 2023
Patras-Med # Project ‘’Herophilus’’: breaking the barriers against pancreatic cancer. # Pancreatic cancer is one of the few cancers for which survival has not substantially improved over the last 25 years. Nowadays, Chimeric Antigen Receptor Therapy (CAR Therapy) has entered clinical practice. It is characterized as “living therapy” and CAR Natural Killer (CAR-NK) cells as “living drugs.” We aim to construct CAR-NK cells derived from induced Pluripotent Stem Cells (iPSCs) through second-generation lentiviral plasmids to bind specifically to Mesothelin (MSLN), a GPI-anchored protein on the membrane of pancreatic cancer cells, inducing immune response.
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October 21, 2023
Peking # Construction of pancreatic cancer-targeted EcN for therapy # Pancreatic cancer is known as the “king of cancer” due to its high malignancy and poor prognosis. The current treatment is far from satisfactory, and the survival rate has not improved significantly in the past 10 years. Among new-rise therapies, bacterial therapy caught our interest. To create a safe and effective strain, Peking 2023 constructed, characterized and modelled an AND-gate-equivalent promoter in EcN to improve the targeting of pancreatic cancer.
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October 21, 2023
PekingHSC # Neuraminidase-boost Influenza Virus to Target Tumors with TFR1 high expression # Cancer is a great threat to human health, and the development of new antitumor therapies is imminent. Studies have shown that TfR1 (transferrin receptor 1) is highly expressed in many types of tumor cells, and it is also found that influenza virus uses the internalization pathway of TfR1 to achieve virus endocytosis, and this process is produced by the interaction between NA protein (neuraminidase) on the surface of the virus and TfR1.
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October 21, 2023
Stuttgart # DentoZym - Biotech for dental health! # Dental caries is the most common health condition worldwide. Improper oral care can lead to biofilm formation on teeth, causing tooth decay. We focus on a new approach regarding caries prophylaxis. A mixture of enzymes, antimicrobial peptides (AMPs) and monolaurin will be used to destruct biofilms and prevent biofilm formation. The enzymes degrade exopolysaccharides, which are crucial for biofilm integrity. Bacteria within the biofilm will be released and eliminated by AMPs and monolaurin, thus preventing biofilm formation.
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October 21, 2023
SUSTech-CHINA # Construction of protective probiotics for precise prevention and control of drug-resistant biofilm # Bacterial biofilm is an environmental adaptation structure formed by microorganisms. After reaching maturity biofilms, intestinal pathogens will develop resistance to antibiotics and host immunity, elicit infection repeatedly, and induce diarrhea or inflammation of the gastrointestinal tract. In mature biofilms, the network structure formed by eDNA is crucial for the maintenance of biofilm stability. The objective of this endeavor was to modify Nissle 1917 so that it could identify Pseudomonas aeruginosa, stimulate the production of recombinant HMGB1 protein and PslG polysaccharide enzyme, degrade the compact structure established by eDNA, and eradicate resistance to biofilm.
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October 21, 2023
SUSTech-MED # Engineered Pseudomonas aeruginosa filamentous phages for biofilm-targeted therapy # We aim to treat P. aeruginosa biofilms by editing a temperate bacteriophage with genes that affect the c-di-GMP pathway and quorum sensing. P. aeruginosa is a common and dangerous pathogen that forms biofilms, which are resistant to antibiotics and regulated by c-di-GMP and AHLs. We inserted wspF, yhjH, aiiA, and ytnP into the pf4 sequence of PAO1, and verified their effects on biofilm formation.
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October 21, 2023
SUSTech-Shenzhen # Peptide Drug Delivery Through Type VI Secretion System # Peptide drugs have become a focus in drug design due to their high specificity, high efficacy, and low immunogenicity. Unfortunately, the nature of peptide drugs themselves causes them to be easily degraded in the internal environment and to have difficulty crossing the cell membrane to function within target cells. Our project attempts to solve this problem. Bacterial secretion systems are diverse, and their primary role is to help bacteria cope with survival pressures, thus obtaining ecological advantages.
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October 21, 2023
SYPHU-China # HER2-Positive Tumor Stalker # In 2023, SYPHU-China focuses on targeted therapy for HER2-positive solid tumors. Protein engineering techniques were used to construct chemoreceptor eTlpC that can target tumor microenvironments with high lactate concentration, and ADC(Antibody-drug conjugate) drugs were used as a means to attack tumors. A synthetic biology approach was utilized to ensure the safety of LBP(Live Biotherapeutic Products) drugs using gene circuits to construct population sensing modules and suicide switch modules.
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