October 21, 2023
Mingdao # Thermostable PCLase-embedded biodegradable PCL plastics # Biodegradable plastics, especially FDA-approved PCL, are becoming increasingly popular as eco-friendly alternatives. However, PCL takes 2-3 years to decompose. Materials embedded with PCL-degrading lipase (PCLase) are gaining interest to address this challenge. Yet, the enzyme activity in the composite cannot tolerate during the thermoforming manufacturing process at 100°C. Mingdao iGEMers employ SpyRing method to enhance thermostability by cyclizing PCLase. This innovative material represents a significant step towards environmental sustainability.
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October 21, 2023
Nanjing-BioX # “Carbon Fast”: Synthetic non-oxidative glycolytic pathway for complete carbon conversion # Global warming, rising sea levels, and continued environmental degradation are all linked to carbon emissions. In China, microbial carbon emissions account for 7% of the total, and most of the carbon emissions generated by microorganisms come from sugar degradation. Carbon sequestration is conducive to reducing carbon emissions. This project attempts to introduce a highly active phosphoketolase (F/Xpk) gene into the heterotrophic C.
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October 21, 2023
Nanjing-BioXstem # – Not provided yet – # – No abstract provided yet –
– まだ要約が提供されていません –
reference:
iGEM Nanjing-BioXstem Team Information iGEM Nanjing-BioXstem Wiki
October 21, 2023
Nanjing-NFLS # Joint Guardian # Osteoarthiritis and Obesity are two major health problems prevalent in our society nowadays. Obesity increases the risk of OA in both weight-bearing (knee) and non-weight-bearing (hand) joints, and obesity doubles the lifetime risk of OA. An efficient new therapeutic target for osteoarthritis related obesity is of urgent need.
骨関節炎と肥満は、現代社会で広範に見られる二つの主な健康問題です。肥満は、体重を支える関節（膝）とそうでない関節（手）の両方で骨関節炎のリスクを増加させ、肥満はOAの一生涯のリスクを二倍にします。骨関節炎に関連する肥満のための効果的な新しい治療ターゲットが緊急に必要です。
reference:
iGEM Nanjing-NFLS Team Information iGEM Nanjing-NFLS Wiki
October 21, 2023
NANJING-NFLSFC # Expression of Cadmium-Binding Proteins & Research on Water Environmental Heavy Metal Pollution Detection Sensor # Today, there are still significant challenges in the detection of heavy metal contamination and its remediation. In this research, we propose a novel, portable, paper-based biosensor for the direct detection of heavy metals such as cadmium in polluted water bodies. This sensor consists of a stem-loop structure containing a cadmium aptamer DNA sequence and the expression and regulation genes for a fluorescent protein.
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October 21, 2023
Nanjing-SDG # Directed Evolution of Clostridium Tyrobutyricum to Improve Butyl Butyrate Production # The increasing depletion of traditional energy and the greenhouse effect pose challenges to global sustainable development. Butyl butyrate’s low viscosity and low carbon emission make it a biofuel with a high potential of alleviating the above problems. We genetically engineered C. tyrobutyricum to enable efficient and large-scale synthesis of butyl butyrate. We optimize metabolic pathways through three strategies to further reinforce the synthesis of butyrate and butanol, including precursor accumulation, inhibition of byproducts, and increasing mass transfer efficiency as well as promoter engineering.
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October 21, 2023
NavarraBG # Biogalaxy Project: Plants as Biosensors # Plants have amazing and significant sensing capabilities. We proposed the use of plants themselves as sensing devices to detect different compounds or environmental conditions. Using plant promoters that are activated in the presence of high concentrations of CO2, selected by the Navarra_BG team, we have produced Arabidopsis plants capable of acting as biosensors against this metabolite. The results obtained open new possibilities for the use of plants as biosensors for other soil pollutant molecules or specific conditions.
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October 21, 2023
NIS-Kazakhstan # Enhanced PET degradation # PET hydrolase (PETase) and mono(2-hydroxyethyl) terephthalate hydrolase (MNETase) are known to synergistically convert PET into its monomeric building blocks. Our team developed six bacterial expression vectors with PETase and six dual expression plasmids containing PETase and MHETase enzymes for optimal PET degradation using Pseudomonas putida, targeting scalability in PET recycling plants. Due to time constraints, we focused on comparing the mutant form of BBa_J435500_W159H_S238F enzyme with wildtype PETase, using p-nitrophenyl butyrate (pNPB) as a PET analog.
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October 21, 2023
NYCEmpireState # Aptamers for the Detection of Stress-Related Salivary Biomarkers # Our motivation was to create an inexpensive and non-invasive aptamer-based approach to assess saliva samples to detect acute stress by clinicians in order to diagnose patients. Through collaboration, we obtained labeled DNA probes with specificity to alpha-amylase. We used an EMSA to visualize the binding of those probes to our protein. We also began work on synthesizing a plasmid to express alpha-amylase in the lab for future experiments.
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October 21, 2023
OTIA-Hangzhou # EGF enhanced Spider Silk Biomaterials for Rapid Wound Healing # Scalds, burns and other accidents often result in skin damage, which brings patients unbearable and long-lasting physical and mental pain, as well as economic pressure. Spider silk is being considered as a potential biomaterial for various biomedical applications. However, farming spiders and extracting silk from them on industrial scale is nearly impossible. Synthetic biology techniques can help produce spider silks recombinantly in different hosts, allowing for the creation of various functional materials depending on the desired application.
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