October 21, 2023
NTU-Singapore # Prime editing optimization and expression in stem cells, bacteria and yeast # Prime editing is a recently developed versatile genome editing technology that offers high efficiency, versatility, and specificity. Unlike traditional methods, it enables precise alterations to genomic DNA without the need for DNA double-strand breaks. Despite numerous optimizations, MMLV RT remains a commonly used reverse transcriptase in prime editing (PE), yet it still exhibits certain limitations that may impede PE efficiency.
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October 21, 2023
NUS-Singapore # Optimised Technique For swiTch Engineering and Ranking (OTTER) # RNA switches are robust and versatile tools in various fields including bioproduction, drug delivery, and disease detection. However, the difficulty in designing the switches hinders its wide applications. A key reason that makes the design process challenging is that the interaction between RNAs are hardly predictable using currently models/tools. Furthermore, there lacks available quality RNA data for developing the models.
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October 21, 2023
Princeton # Stick and Secrete: Building an Interkingdom Communication Pathway Between Bacteria and Mammalian Cells # We aim to develop a novel “stick-and-secrete” bacterial-mammalian cell communication system where engineered bacteria selectively adhere to mammalian cells, then secrete a ligand to elicit a particular response in the mammalian cell. This project is designed to be a modular toolbox that can provide a diverse array of highly localized responses in mammalian cells for scientific research.
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October 21, 2023
SCU-BSE-China # YEAST PATRONUS: Polyamines Assist TheRmO and NatUral Support # Biomanufacturing, characterized by its green and efficient features, is poised to be the future production method to replace traditional chemical manufacturing. Yeast cell factory has been widely applied in practical production. However, multiple stress challenges(such as high temperature and extreme pH environments) during the fermentation process significantly reduce its availability. Enhancing its tolerance can substantially reduce production costs and energy consumption, expanding the application scope of yeast cell factories.
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October 21, 2023
Sheffield # PARSE: PhAge deRived growth SlowErs # Molecular biology workflow is significantly impacted by microbial dynamics, which itself is heavily dependent on cell growth rate. To date, an automatic plasmid system in which growth rate can be reliably predicted and instigated has not yet been developed. Such a system would have several useful applications in bioproduction, co-culturing and directed evolution. Thus, our goal is to create a system that allows for Escherichia coli growth rate to be a genetically programmable parameter.
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October 21, 2023
TAU-Israel # CtrlC - Controlling Plasmids Copy Number # Plasmids constitute a key tool in synthetic biology offering a versatile platform for a variety of research and industrial applications. The copy number (CN) is a fundamental feature of plasmids which affects both the production rate of the proteins encoded on the plasmid and the metabolic burden it induces on the host cell.
プラスミドは合成生物学において重要なツールであり、多種多様な研究や産業応用のための多機能プラットフォームを提供します。コピーナンバー（CN）はプラスミドの基本的特性であり、プラスミド上でエンコードされたタンパク質の生産速度や、宿主細胞に課す代謝負荷の両方に影響を与えます。
reference:
iGEM TAU-Israel Team Information iGEM TAU-Israel Wiki
October 21, 2023
UBC-Vancouver # PILOT: Platformed Inteins: A linked orthogonal toolkit # Protein-based therapeutics have been approved for clinical use due to their unique structural and functional properties. However, high throughput production of novel therapeutic proteins is hindered by cellular cytotoxicity and resource intensity of conventional manufacturing methods, entailing slow turnaround times. Synthetic biology technologies have emerged to eliminate such barriers to whole-cell protein production in vitro by harnessing cell-free protein synthesis (CFPS).
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October 21, 2023
UCAS-China # NOX: Neo-quOrum sensing-based Xpression biosensor platform # Trace substance detection remains challenging, especially regarding food safety and medical care in daily practice. While it is possible to carefully examine substances with great accuracy in labs, it’s usually too costly or inconvenient to conduct it in everyday life. We chose BPA and bile salts as examples, demonstrating the significance and the necessity of employing portable and scalable biosensors in daily situations.
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October 21, 2023
UIncheon # Symbionex : Quorum sensing based biocontainment system for eukaryotic cell in coculture condition # This year, our team has developed a biocontainment system for eukaryotic cells, particularly designed for yeast (S. cerevisiae) in coculture conditions, utilizing bacterial quorum sensing and a toxin-antitoxin system. This biocontainment system functions as a conditional kill switch, reliant on the concentration of AHL signal molecule from a bacterial quorum sensing. We have also strived to present the prospective applications of our biocontainment system.
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October 21, 2023
USP-Brazil # Strep-by-Strep # Our current project is called “St(r)ep by St(r)ep”. Streptomyces are a type of bacteria that are very important for human health, as they produce almost half of all the antibiotics used today, but much of their potential is not exploited because the genes responsible for producing these compounds are encrypted in their DNA.
私たちの現在のプロジェクトは「St(r)ep by St(r)ep」という名称です。ストレプトマイセスは、今日使用されている抗生物質のほぼ半分を生産するという、人間の健康に非常に重要なバクテリアの一種です。しかし、これらの化合物を生産するための遺伝子がそのDNAに暗号化されているため、その潜在能力の多くが未だに活用されていません。
reference:
iGEM USP-Brazil Team Information iGEM USP-Brazil Wiki