October 21, 2023
VITVellore # PETrify: Unbottling the Future Using FAST-PETase-mutated MHETase Combinations # To tackle the growing problem of PET bottle accumulation in Indian waste disposal systems and the health hazards leached PET microplastics possess, we adopted a combination approach of FAST-PETases and mutated MHETases to degrade PET plastics. Via in-silico studies, we generated the single and double mutant MHETase (S491A and S196A,T434S respectively). In comparison to the wild type MHETase, they were comparatively more stable.
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October 21, 2023
WLC-Milwaukee # The Dirt Detective: A bioavailable phosphorus sensor powered by E. coli # Agriculture is one of the biggest industries in Wisconsin, where our team is based, and the crops produced here go all over the world. One important component in agricultural success is soil composition. It is essential to have the appropriate nutrients in the soil for crops to grow and provide a maximal return. Our team has engineered Escherichia coli to sense bioavailable phosphate in soil and provide a colorimetric readout of concentration.
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October 21, 2023
WrightState-OH # Deconta-Mn-ate: Advancing Manganese Detection # The iGEM-WrightState Deconta-Mn-ate research project combines cutting-edge research, social outreach and collaboration to address the important issue of manganese contamination in drinking water. Our goal is to provide innovative solutions for water testing and to inspire scientists and citizens to join us in this vital cause. Our project builds upon our 2022 E. coli-based biosensor capable of detecting manganese down to 0.5ppm. Our 2023 team adapted the biosensor to a cell-free approach, reducing assay time to 2 hours.
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