October 21, 2023
JLU-China # EyeGuard: A System for Inhibiting Pseudomonas aeruginosa Using DMBT1 Protein Generation and Hardware Platform # Contact lenses, while correcting vision and enhancing aesthetics, pose hygiene risks, including Pseudomonas aeruginosa infection. Our iGEM project addresses this by engineering E. coli into lens solutions, creating a DMBT1 protein system to inhibit Pseudomonas growth, reducing infection risks. This system comprises a toxin-antitoxin system, population sensing system for bacterial growth control, and an Hsp27 self-inducing system for increased protein production.
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October 21, 2023
SUSTech-CHINA # Construction of protective probiotics for precise prevention and control of drug-resistant biofilm # Bacterial biofilm is an environmental adaptation structure formed by microorganisms. After reaching maturity biofilms, intestinal pathogens will develop resistance to antibiotics and host immunity, elicit infection repeatedly, and induce diarrhea or inflammation of the gastrointestinal tract. In mature biofilms, the network structure formed by eDNA is crucial for the maintenance of biofilm stability. The objective of this endeavor was to modify Nissle 1917 so that it could identify Pseudomonas aeruginosa, stimulate the production of recombinant HMGB1 protein and PslG polysaccharide enzyme, degrade the compact structure established by eDNA, and eradicate resistance to biofilm.
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