plastic-degradation

Bioplus-China

October 21, 2023
plastic-degradation, petase-spider-silk-fusion-protein, prokaryotic-expression-system
high-school

Bioplus-China # “Eating plastic spider silk”- Improving plastic degradation with PETase-Spider Silk fusion protein # Plastic waste poses a serious challenge to the ecological environment. Enzymatic degradation offers one potentially green way to achieve the recycling and utilization of plastic waste. Poly (ethylene terephthalate) (PET) is one of the most abundantly produced synthetic polymers, making up 12% of global solid waste. Enhancing the “capture and gathering” of plastics is crucial for increasing the hydrolysis efficiency of PET hydrolase degradation. ...

Heidelberg

October 21, 2023
bioremediation, pseudomonas-fluorescens-co-culture, plastic-degradation
bioremediation

Heidelberg # RemixHD: a novel bioremediation method using a Pseudomonas fluorescens co-culture for enzymatic mixed plastic degradation # Our ReMixHD platform emerges as a novel method for upcycling mixed plastic using a co-culture of genetically modified Pseudomonas fluorescens. We introduced polyethylene terephthalate and polyethylene degrading enzymes. Two in-vivo biosensors can control the expression of a growth factor depending on the concentration of the monomers, terephthalic acid and alkanes. The main strain uses terephthalic acid as a carbon source to synthesize a recombinant product. ...

WUST-China

October 21, 2023
plastic-degradation, microbial-oil-production, petase-and-mhetase
bioremediation

WUST-China # Plastic Killer # WUST-China incorporated PETase and MHETase into Rhodococcus rhodococcus PD630 to break down PET and yield ethylene glycol (EG) and terephthalic acid (TPA). Additionally, they explored various promoters, signal peptides, and enhancers to enhance the breakdown of PET plastics. At the same time, the method introduced 1,2 dioxygenase to transform degradation-generated TPA into protocatechuic acid PCA, thus enabling Rhodococcus rhodococcus to effectively use PET and accumulate microbial lipids. ...