NTU-Singapore

October 21, 2023

NTU-Singapore # Prime editing optimization and expression in stem cells, bacteria and yeast # Prime editing is a recently developed versatile genome editing technology that offers high efficiency, versatility, and specificity. Unlike traditional methods, it enables precise alterations to genomic DNA without the need for DNA double-strand breaks. Despite numerous optimizations, MMLV RT remains a commonly used reverse transcriptase in prime editing (PE), yet it still exhibits certain limitations that may impede PE efficiency. ...