nissle-1917

GEC-Guangzhou

October 21, 2023

nissle-1917, colorectal-cancer-therapy, cytosine-deaminase

high-school

GEC-Guangzhou # Targeted therapy for colorectal cancer # We used Nissle 1917 as the chassis microbe because it is safe, reliable and easy to edit for the human body. We designed two modules of targeting and therapy. For the targeted part, we fusion expression of INP and HIPA genes, INP can anchor HIPA to the cell wall of engineered bacteria, and HIPA protein can recognize and bind to the receptor protein on the surface of cancer cells. ...

Zhejiang-United

October 21, 2023

crispr-cas9, genome-editing-tool, nissle-1917

high-school

Zhejiang-United # Crisprologists: Optimized High-Efficiency Genome Editing Tool based on CRISPR-Cas9 System # When using CRISPR-Cas for gene editing, it is common to encounter cells that do not undergo the expected edits, thereby reducing the efficiency of CRISPR-Cas gene editing. To address this issue, we have employed a strategy of increasing the copy number of Cas9 to construct an efficient genome editing tool based on the CRISPR-Cas9 system for Nissle 1917. ...