NIS-Kazakhstan

October 21, 2023

NIS-Kazakhstan # Enhanced PET degradation # PET hydrolase (PETase) and mono(2-hydroxyethyl) terephthalate hydrolase (MNETase) are known to synergistically convert PET into its monomeric building blocks. Our team developed six bacterial expression vectors with PETase and six dual expression plasmids containing PETase and MHETase enzymes for optimal PET degradation using Pseudomonas putida, targeting scalability in PET recycling plants. Due to time constraints, we focused on comparing the mutant form of BBa_J435500_W159H_S238F enzyme with wildtype PETase, using p-nitrophenyl butyrate (pNPB) as a PET analog. ...