October 21, 2023
GEC-CHINA # Directed evolution to elevate bio-production of NAD+ for anti-ageing # As the aging population continues to grow, the treatment of aging and age-related diseases has become an important societal issue. NAD+ is a critical molecule in cellular metabolism and life span, but its levels decline with age. NAMPT is the key enzyme for NAD+ synthesis, however, the catalytic activity of human NAMPT is extremely low. Therefore, our project aims to harness the power of directed evolution to select the NAMPT mutant form with superior activity and accelerate the bio-production of NAD+ and its application in anti-aging therapy.
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October 21, 2023
Nanjing-SDG # Directed Evolution of Clostridium Tyrobutyricum to Improve Butyl Butyrate Production # The increasing depletion of traditional energy and the greenhouse effect pose challenges to global sustainable development. Butyl butyrate’s low viscosity and low carbon emission make it a biofuel with a high potential of alleviating the above problems. We genetically engineered C. tyrobutyricum to enable efficient and large-scale synthesis of butyl butyrate. We optimize metabolic pathways through three strategies to further reinforce the synthesis of butyrate and butanol, including precursor accumulation, inhibition of byproducts, and increasing mass transfer efficiency as well as promoter engineering.
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