crispr/cas9-system

Shanghai-BioX

October 21, 2023
high-purity-amylopectin, crispr/cas9-system, starch-based-biodegradable-plastic-production
high-school

Shanghai-BioX # Starch shaper: Biosynthesis of high-purity amylopectin for high-performance bioplastics # In order to obtain high-purity amylopectin, the starch synthesis pathway in the plant sweet potato (Ipomoea batatas) was modified using CRISPR/Cas9 system. Firstly, sgRNA oligos targeting the IbGBSSI gene was designed and annealed, and was inserted into the plasmid psgR-Cas9-At to construct the backbone vector, which was combined with the binary vector pCAMBIA1301s to obtain the expression vector. ...