breast-cancer-detection

CJUH-JLU-China

October 21, 2023
breast-cancer-detection, mir-21-5p-biomarker, crispr/cas13a-system
diagnostics

CJUH-JLU-China # BreFast: A Fast and Convenient Screening Kit for Breast Cancer Early Detection # While breast cancer is the cancer with the highest incidence rate among women, early detection reduces the mortality rate of breast cancer effectively. Therefore, we aim to create a fast and convenient screening kit, BreFast, for breast cancer early detection. We employed comprehensive bioinformatics methods to identify a breast cancer-associated miRNA, miR-21-5p, as an optimal biomarker for screening, and engineered a CRISPR/Cas13a system that could be specifically activated by miR-21-5p to cleave any by-stander RNA such as a dual-labeled reporter RNA. ...