ancestral-sequences-reconstruction

NNU-CHINA

October 21, 2023
pet-depolymerization, machine-learning-algorithms, ancestral-sequences-reconstruction
bioremediation

NNU-CHINA # Machine learning-aided engineering of two-enzyme system for PET depolymerization # One of the grand challenges of the 21st century is to solve the environmental problem caused by plastic waste. In 2016, researchers identified PETase that can depolymerize PET into monomers. However, PETase was limited by temperature ranges. To overcome this challenge, we reconstructed ancestral sequences of PETase by employing the profile-HMM algorithm. To further enhance the thermostability and catalytic efficiency of ASR-PETase, we used machine learning algorithms to identify stabilizing variants. ...