Fujian-United #

Synthesis of new feed additives through genetic engineering to express phytase with AppA and An_phy33 #

The increasing scale of global livestock farming has led to a shortage in the supply of conventional feed protein raw materials in the feed industry and even the phenomenon of competition with people for food, livestock farming and processing are also prone to environmental problems such as water and soil pollution.We construct recombinant plasmids pYES2-AppA and pYES2-An_phy33, transform them into E.coli DH5α then transform into Saccharomyces cerevisiae BY4741 and fermentate it to express phytase. Application of yeast single-cell proteins from phytase can be added to livestock feed to improve nutrient absorption by animals.

世界的な畜産の拡大に伴い、飼料業界の従来の飼料タンパク質の原料供給が不足し、人間と食糧を競う現象さえも発生しています。また、畜産や加工は水汚染や土壌汚染などの環境問題を引き起こす可能性があります。私たちは、組換えプラスミドであるpYES2-AppAとpYES2-An_phy33を作成し、それらをE.coli DH5αに変換し、その後Saccharomyces cerevisiae BY4741に変換し、それを発酵させてフィターゼを発現させます。フィターゼ由来の酵母単細胞タンパク質の利用は、畜産飼料に添加することで動物による栄養吸収を向上させることができます。

reference:

• iGEM Fujian-United Team Information
• iGEM Fujian-United Wiki